The Molisch test is based on the dehydration of sugar molecules to form furfural derivatives when carbohydrates are heated in the presence of a concentrated acid (usually sulfuric acid or hydrochloric acid). These furfural derivatives then react with α-naphthol (or another phenolic compound) present in the Molisch reagent to form a purple-colored compound. This color change is indicative of a positive test for carbohydrates.
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