If you are analyzing large molecules or late-eluting peaks with broad shapes, switch your response time to 2.0 seconds or higher. This reduces electrical noise without sacrificing peak shape. The manual explains how to change it; the "better" part is knowing when to change it.
The Shimadzu SPD-20A is a versatile UV-Vis detector designed for HPLC systems, offering high sensitivity and stability. Its features include: shimadzu spd20a user manual better
If you notice a drifting baseline or ghost peaks, your flow cell may be contaminated. Flush it with a suitable solvent (like 2-propanol) if you've been using salts or buffers. If you are analyzing large molecules or late-eluting
Shimadzu provides a vague "maintain as needed" statement. Here is an aggressive, practical schedule for . practical schedule for .