Before full cosmid prep, PCR is used to screen pooled clones. A typical shows:
Understanding Cosmids: The DNA Delivery Vans of Biotechnology cosmid pics
: Usually for ampicillin, used to identify successful clones. Multiple Cloning Site (MCS) : Where your target DNA is inserted. Before full cosmid prep, PCR is used to screen pooled clones
Because cosmids are large (40-50 kb), their contour length in EM pics is significantly longer than that of a plasmid. A trained eye can measure the length of the DNA in the picture and calculate the exact base pair count (1 kb = approximately 0.34 micrometers of contour length). Before full cosmid prep