Primer3 0.4.0 • Reliable & Trusted

The percentage of nitrogenous bases that are either Guanine or Cytosine. Primer Length: Typically ranging from 18 to 30 base pairs.

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For the uninitiated, Primer3 is a widely used open-source software program for designing PCR primers. It was originally developed by the Whitehead Institute and the Howard Hughes Medical Institute. Its goal is simple yet complex: take a DNA sequence (the source) and find a pair of primers that will amplify a specific region with high efficiency and specificity. primer3 0.4.0